BCA Protein Assay is a reliable method used to measure the total amount of protein in a sample. It’s based on a color change that occurs when proteins react with special reagents containing copper. The purple color produced is directly related to the protein concentration and is measured using a plate reader or spectrophotometer. This assay is known for its high accuracy, compatibility with many buffers, and longer color stability compared to other protein tests. In biotechnology labs, the BCA assay is commonly used during protein purification, sample prep, and quality control steps to ensure consistent and accurate protein levels.